As world fighting and understanding the novel Coronavirus , how it spreads and evolves ,genetic sequencing has provided enormous insight. Though many of these mutations does not affect the viruses in meaningful ways, but they are important for understanding everything how it moves through the population globally to where a person might has been infected locally. NGS is a powerful tool for tracking the mutations and monitoring a patient infection progression and immune response. NGS remains a powerful crucial evidence to public health officials, vaccine and drug developers and researchers enabling laboratories to
1) To monitors the virus transmission destinations globally
2) Easy way to identify mutations to limit the occurrence of new types of strains
3) Define viral mutations that by defind molecular diagnostic assays can prevent detection
4) Classify viral mutations that can influence the potency of vaccines
5) Screen targets for probable therapeutics for Covid 19
6) Isolate and classify respiratory coinfections and alleles of antimicrobial resistance.
NGS technology have successfully sequenced emerging B 1.1.7 (UK) and B 1.351 (South Africa) genomes now available in the GISAID repository. It identify novel variants, tracking transmission, detects mutations and identify the coinfections and complex diseases. Four major steps are involved in NGS – Library preparation, cluster preparation, sequencing and alignment and data analysis